About Mouse Monoclonal IgG1 Anti-CD138 (Syndecan-1) [B-A38]

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Litterature1. Borset M., et al. (1993) Lack of IL-1 secretion from human myeloma cells highly purified by immunomagnetic separation. Br. J. Haem. 85; 446 ff. 2. Wijdenes J., et al. (1996) A plasmocyte selective monoclonal antibody (B-B4) recognises Syndecan-1. Br. J. Haem. 94; 318-323. 3. Inki. P. and Jalkanen M. (1996) The role of syndecan-1 in malignancies. Ann. Med. 28(1); 63-67. 4. Anttonen A., et al. (1999) Syndecan-1 expression has prognostic significance in head and neck carcinoma. Brit J. Cancer 79; 558-564. 5. Yang Y, MacLeod V, Dai Y, Khotskaya-Sample Y, Shriver Z, Venkataraman G,Sasisekharan R, Naggi A, Torri G, Casu B, Vlodavsky I, Suva LJ, Epstein J,Yaccoby S, Shaughnessy JD Jr, Barlogie B, Sanderson RD. (2007) The syndecan-1 heparan sulfate proteoglycan
TestMouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Tips*Gentaur's antibodies are intended for in vitro research use only. They must not be used for clinical diagnostics and not for in vivo experiments in humans or animals. ** The preservative sodium azide is known to be poisonous and potentially hazardous to health. It should be handled only by trained staff. Despite of the product's low azide concentration it must be handled with care. Dispose according to regional rules!
AboutMonoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Product presentationAntibody solution in stabilizing phosphate buffer pH 7.3. Contains 0.09 % sodium azide**and 0.8% BSA . The volume is sufficient for at least 100 immunohistochemical tests (100 µl working solution / test). Use appropriate antibody diluent e.g. Gentaur Art .No. PU002.
Recommended secondary reagentWe recommend the use of Gentaur's Universal Staining System DAB (Art. No. DA005) or AEC (Art.-No. AE005), if higher sensitivity is required VECTASTAIN Elite ABC (Art. No. PK-6102) or ABC AP (Art. No. AK-5002) systems are applicable.
DescriptionThis antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
PretreatmentNo protease necessary for IHC, but heat pre-treatment with citrate buffer (Art. No. DE000) or EDTA buffer pH 9 (Art. No. DE006) may enhance staining intensity.
AvailabilityPlease contact us to check the availability
Species reactivityHuman, others not tested
UniProt numberGo to UniProt/SwissProt
SpecificityHuman CD138, Syndecan 1
Product is supplied asliquid concentrate
Product categoryPrimary Antibody
Tested applicationsIHC(C, P), FACS
Storage methodStore at 2-8°C
ImmunogenU266 cell line
Latin nameMus musculus
Recommended concentration for use1:20-1:100
Protein concentration50 µg/ml
Positive controlTonsil
Antibody's cloneB-A38
Antibody's raised inMouse
Antibody's isotypeIgG1