About Human SDC1/Syndecan-1 PicoKine ELISA Kit

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1x96 well plate

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More gene infoSyndecan 1, also known as SYND1 or CD138, is a protein which in humans is encoded by the SDC1 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. This gene is mapped to 2p24.1. The syndecans mediate cell binding, cell signaling, cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan 1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. What’s more, Syndecan-1 is also a sponge for growth factors, with binding largely via heparan sulfate chains.
ELISA kit descriptionGENTAUR's human Syndecan-1 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Syndecan-1 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, Q23-G254) and your samples are measured with a biotin conjugated polyclonal from goat specific for Syndecan-1 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Syndecan-1 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- human Syndecan-1 antibodies;1 ST0000-10;Lyophilized recombinant human Syndecan-1 standard;10ng/tube×2 AR1107;Biotinylated anti- human Syndecan-1 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
How the elisa worksSample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human Syndecan-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant or serum to the ELISA plate. Keep in mind that each human Syndecan-1 standard solution and each sample be measured in duplicate.
ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (ng/ml);1.23;3.25;5.87;1.32;4.05;6.42 ELISA standard deviations 0.048;0.15;0.299;0.07;0.235;0.417 CV(%);3.9;4.6;5.1;5.3;5.8;6.5
ELISA standard curveConcentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.015 + 0.182 + 0.253 + 0.423 + 0.746 + 1.355 + 1.827 + 2.310
Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
ELISA's applicationFor quantitative measurement of human Syndecan-1 in cell culture supernatant and serum.
Detector and capure antibodiesa monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
AlternativeCD138 ; P18827 ; SDC1 ; SYND1 ; Synd-1 ; Synd1 ; syndecan1 ; Syndecan-1
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Target of this ELISANatural and recombinant human Syndecan-1
ELISA kit detection limits156pg/ml to 10000pg/ml
Gene testedhuman Syndecan-1
AntigenNSO, Q23-G254
Gene namesyndecan 1
ELISA's sensitivity<10pg/ml
Gen protein identityP18827
Minutes of TMB incubation25-30
Entrez nr.6382