About Human Syndecan-4/SDC4 PicoKine ELISA Kit

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1x96 well plate

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Detail Description
ELISA kit description GENTAUR's human Syndecan-4 ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibodies from mouse specific for Syndecan-4 was used as capure antibodies on the ELISA test plate. The internal standards (NSO, E19-E145) and your samples are measured with a biotin conjugated polyclonal from goat specific for Syndecan-4 is added subsequently and then followed by washing with PBS or TBS buffers. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffers. HRP substrate TMB are used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Syndecan-4 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
More gene info Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What’s more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
The ELISA contains Catalog number;Description;Quantity -;96-well plate precoated with anti- human Syndecan-4 antibodies;1 ST0000-10;Lyophilized recombinant human Syndecan-4 standard;10ng/tube×2 AR1107;Biotinylated anti- human Syndecan-4 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
How the elisa works Sample the amount of 100ul/well of the standard solution at 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml human Syndecan-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant or serum to the ELISA plate. Keep in mind that each human Syndecan-4 standard solution and each sample be measured in duplicate.
ELISA's precision parameters intra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);630;1447;2135;718;1604;2369 ELISA standard deviations 26.5;83.9;100.3;35.2;104.3;120.8 CV(%);4.2;5.8;4.7;4.9;6.5;5.1
Alternative Amphiglycan;MGC22217;OTTHUMP00000031788;ryudocan amphiglycan;Ryudocan;Ryudocan core protein;SDC 4;SDC4;SDC4_HUMAN;SYND 4;SYND4;syndecan 4;syndecan 4 (amphiglycan, ryudocan);syndecan proteoglycan 4;Syndecan-4;Syndecan4
ELISA standard curve Concentration(pg/ml) + 0 + 62.5 + 125 + 250 + 500 + 1000 + 2000 + 4000 O.D. + 0.006 + 0.060 + 0.129 + 0.256 + 0.442 + 0.829 + 1.384 + 1.979
Keep at Store at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reaction the ELISA does not cross react with any other proteins except from closely related species
ELISA's application For quantitative measurement of human Syndecan-4 in cell culture supernatant and serum.
Detector and capure antibodies a monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Target of this ELISA Natural and recombinant human Syndecan-4
ELISA kit detection limits 62.5pg/ml to 4000pg/ml
Gene tested human Syndecan-4
Antigen NSO, E19-E145
Uniprot Syndecan-4
Gene name syndecan 4
ELISA's sensitivity < 10pg/ml
Gen protein identity P31431
Minutes of TMB incubation 15-20
Entrez nr. 6385
Anti-coagulation /