About Human SDC1 (Syndecan 1) ELISA Kit

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96 Tests

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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human SDC1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Human SDC1. The concentration of Human SDC1 in samples can be calculated by comparing the OD of the samples to the standard curve.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
Research AreaCancer, Metabolism, Neuroscience, Signal transduction, Stem cells
Cross ActivityNo significant cross-reactivity or interference was observed.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
Shelf lifeUse Human SDC1 (Syndecan 1) ELISA Kit before 6 months
ApplicationFor research use only. Not for diagnostic procedures.
Storage TemperatureShort term: 4°C; Long term: see manual.
Sample TypeSerum, Plasma, Biological Fluids
Estimated Turnaround Time6-11 business days
Detection Range0.16-10 ng/mL
Detection MethodColorimetric
Assay Time4.5 hours
Sensitivity0.1 ng/mL
Shipping ConditionsIce packs
Assay TypeSandwich
Species ReactivityHuman
Target's alterntive nameSDC1
Target NameNA