About Rat SDC1 (Syndecan 1) ELISA Kit

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Detail Description
Test Principle This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDC1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDC1. The concentration of Rat SDC1 in samples can be calculated by comparing the OD of the samples to the standard curve.
About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Precaution of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Research Area Cancer, Metabolism, Neuroscience, Signal transduction, Stem cells
Cross Activity No significant cross-reactivity or interference was observed.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality Systems The kit is manufactured at ISO 9001 certified facilities.
Application For research use only. Not for diagnostic procedures.
Shelf life Use Rat SDC1 (Syndecan 1) ELISA Kit before 6 months
Storage Temperature Short term: 4°C; Long term: see manual.
Sample Type Serum, Plasma, Biological Fluids
Estimated Turnaround Time 6-11 business days
Latin name Rattus norvegicus
Detection Range 1.56-100 ng/mL
Detection Method Colorimetric
Sensitivity 0.94 ng/mL
Shipping Conditions Ice packs
Assay Time 4.5 hours
Assay Type Sandwich
Target's alterntive name SDC1
Species Reactivity Rat
Target Name NA
Specificity NA
Stability NA
Precision NA