About Rabbit SDC1 (Syndecan 1) ELISA Kit

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96 Tests

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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit SDC1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit SDC1. The concentration of Rabbit SDC1 in samples can be calculated by comparing the OD of the samples to the standard curve.
AboutRabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
Research AreaCancer, Metabolism, Neuroscience, Signal transduction, Stem cells
Cross ActivityNo significant cross-reactivity or interference was observed.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
Shelf lifeUse Rabbit SDC1 (Syndecan 1) ELISA Kit before 6 months
ApplicationFor research use only. Not for diagnostic procedures.
Storage TemperatureShort term: 4°C; Long term: see manual.
Sample TypeSerum, Plasma, Biological Fluids
Latin nameOryctolagus cuniculus
Estimated Turnaround Time6-11 business days
Detection Range0.16-10 ng/mL
Detection MethodColorimetric
Shipping ConditionsIce packs
Assay Time4.5 hours
Sensitivity0.1 ng/mL
Assay TypeSandwich
Species ReactivityRabbit
Target's alterntive nameSDC1
Target NameNA