About Rat SDC4(Syndecan 4) ELISA Kit

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Size

96T

Catalog no

ELK6004

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Details

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Detail Description
Test principle The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 4 (SDC4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 4 (SDC4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 4 (SDC4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 4 (SDC4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
About Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Research Area Signal transduction;Infection immunity;Bone metabolism;Rheumatology;Immunodeficiency;
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Alternative Names SYND4; Amphiglycan; Ryudocan; Ryudocan core protein
Latin name Rattus norvegicus
Detection range 31.25-2000pg/mL
Sensitivity 13.8pg/mL
Standard 2000pg/mL
Assay Type Sandwich
Assay length 3h