About Human Syndecan 3 (SDC3) ELISA Kit, 96 well plate

The following list shows the most important features of our product:

Size

96T

Catalog no

EKU07551

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Details

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DetailDescription
Assay principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 3 (SDC3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 3 (SDC3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 3 (SDC3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 3 (SDC3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
DescriptionA microtiter plate (spelled Microtiter is a registered trade name in the United States) or microplate or micro well plate or multiwell, is a flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories. A very common usage is in the enzyme-linked immunosorbent assay (ELISA), the basis of most modern medical diagnostic testing in humans and animals. A microplate typically has 6, 24, 96, 384 or 1536 sample wells arranged in a 23 rectangular matrix. Some microplates have even been manufactured with 3456 or 9600 wells, and an "array tape" product has been developed that provides a continuous strip of microplates embossed on a flexible plastic tape.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
ELISA's stabilityThe stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Precision of the testIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syndecan 3 (SDC3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syndecan 3 (SDC3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV
WarningsThe Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
Storage recommendation-20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
ELISA's specificityThis assay has high sensitivity and excellent specificity for detection of Syndecan 3 (SDC3). No significant cross-reactivity or interference between Syndecan 3 (SDC3) and analogues was observed.
ELISA's cross-reactivityThis assay doesn't seem to cross-react with other species. For more information about cross-reactivity please contact us.
QCThe Kit is manufactured at ISO 9001 and ISO 13485 certified facilities.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
NotesFor research use only. Not for diagnostic procedures.
ProtocolPlease see ELISA's datasheet, otherwise contact us
Alternate gene nameSDCN; SYND3; N-Syndecan; Syndecan Proteoglycan 3
Samples to be analyzedTissue homogenates and other biological fluids.
Alternate protein numberPlease refer to SwissProt
Protein numberPlease refer to Uniprot
Gene numberPlease refer to GenBank
Verified reactivityHomo sapiens (Human)
Estimated production time7-11 business days
Research main areaTumor immunity;
Detection limits0.625-40 ng/mL
ELISA detectionColorimetric
Sensitivity limit0,226 ng/mL
Gene nameSyndecan 3
Assay classSandwich
Use for8 months
Shipping requirementsBlue ice
Assay duration3 hours
Verified applicationsELISA