About Human Syndecan-3/SDC3 PicoKine ELISA Kit

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Size

1x96 well plate

Catalog no

EK1555

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DetailDescription
More gene infoSyndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
ELISA kit descriptionGENTAUR's human Syndecan-3 ELISA test is a ready to use ELISA kit. We use a monoclonal from mouse specific for Syndecan-3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A41-V387) and your samples are measured with a biotin conjugated polyclonal from goat specific for Syndecan-3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more human Syndecan-3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
How the elisa worksSample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml human Syndecan-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of human cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each human Syndecan-3 standard solution and each sample be measured in duplicate.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- human Syndecan-3 antibodies;1 ST0000-10;Lyophilized recombinant human Syndecan-3 standard;10ng/tube×2 AR1107;Biotinylated anti- human Syndecan-3 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);177;695;3112;186;987;4569 ELISA standard deviations 5.84;25.02;108.92;6.32;37.50;164.48 CV(%);3.3;3.6;3.5;3.4;3.8;3.6
ELISA's applicationFor quantitative measurement of human Syndecan-3 in cell culture supernatant , cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
ELISA standard curveConcentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.011 + 0.030 + 0.063 + 0.124 + 0.282 + 0.656 + 1.353 + 2.398
AlternativeN-syndecan ; Sdc3 ; SDC3_HUMAN ; SDCN ; SYND3 ; Syndecan neural type ; syndecan proteoglycan 3 ; Syndecan-3 ; Syndecan 3 ; O75056
Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
Detector and capure antibodiesa monoclonal antibodies from mouse;a biotinylated polyclonal antibodies from goat
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Target of this ELISANatural and recombinant human Syndecan-3
ELISA kit detection limits156pg/ml to 10000pg/ml
Gene testedhuman Syndecan-3
Anti-coagulationheparin or EDTA
AntigenNSO,A41-V387
UniprotSyndecan-3
Gene namesyndecan 3
ELISA's sensitivity<10pg/ml
Gen protein identityO75056
Minutes of TMB incubation20-25
Entrez nr.9672