About Mouse Syndecan-3/SDC3 PicoKine ELISA Kit

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Size

1x96 well plate

Catalog no

EK1556

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DetailDescription
More gene infoSyndecan-3 is a protein that in humans is encoded by the SDC3 gene. The protein encoded by this gene belongs to the syndecan proteoglycan family, which was assigned to human chromosome 1p35.2, and it showed that in the mouse the Synd3 gene maps to chromosome 4 near the Lmyc gene. The transfection of SDC3 resulted in the formation of long filopodia-like structures, microspikes, and varicosities in several cell lines. Besides, the gene plays a role in the organization of cell shape by affecting the actin cytoskeleton, possibly by transferring signals from the cell surface in a sugar-dependent mechanism. Allelic variants of this gene have been associated with obesity.
ELISA kit descriptionGENTAUR's mouse Syndecan-3 ELISA test is a ready to use ELISA kit. We use a monoclonal from rat specific for Syndecan-3 has been precoated onto 96-well plates. Standards (Expression system for standard: NSO, Immunogen sequence: A45-V387) and your samples are measured with a biotin conjugated polyclonal from goat specific for Syndecan-3 will than be washed with PBS. The colour reaction with TMB on HRP will colour the well from blue to yellow with the stop solution. The more mouse Syndecan-3 in your sample the higher the ELISA signal will be compared to this elisa's standard curve.
How the elisa worksSample the amount of 100ul/well of the standard solution at 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Syndecan-3 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffers into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatant, cell lysate, tissue homogenates, serum or plasma (heparin, EDTA) to the ELISA plate. Keep in mind that each mouse Syndecan-3 standard solution and each sample be measured in duplicate.
The ELISA containsCatalog number;Description;Quantity -;96-well plate precoated with anti- mouse Syndecan-3 antibodies;1 ST0000-10;Lyophilized recombinant mouse Syndecan-3 standard;10ng/tube×2 AR1107;Biotinylated anti- mouse Syndecan-3 antibodies;130μl(dilutions 1:100) AR1103;Avidin-Biotin-Peroxidase Complex (ABC);130μl(dilutions 1:100) AR1106-1;Sample diluent buffers;30 ml AR1106-2;antibodies diluent buffers;12ml AR1106-3;ABC diluent buffers;12ml AR1104;TMB color developing agents;10ml AR1105;TMB stop solution;10ml -;Adhesive cover;4
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
ELISA's precision parametersintra assay precisions and inter assay precisions samples;1;2;3;1;2;3 n;16;16;16;24;24;24 mean (pg/ml);205;899;2750;245;1010;4290 ELISA standard deviations 7.99;39.55;115.50;10.78;59.59;304.59 CV(%);3.9;4.4;4.2;4.4;5.9;7.1
ELISA's applicationFor quantitative measurement of mouse Syndecan-3 in cell culture supernatant , cell lysate, tissue homogenates, serum and plasma heparin, EDTA .
ELISA standard curveConcentration(pg/ml) + 0 + 156 + 312 + 625 + 1250 + 2500 + 5000 + 10000 O.D. + 0.001 + 0.092 + 0.176 + 0.330 + 0.649 + 1.135 + 1.830 + 2.408
AlternativeN-syndecan ; Sdc3 ; SDC3_HUMAN ; SDCN ; SYND3 ; Syndecan neural type ; syndecan proteoglycan 3 ; Syndecan-3 ; Syndecan 3 ; O75056
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Keep atStore at 4˚C for 6 months, at -20˚C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
Detector and capure antibodiesA rat monoclonal detector antibodies is used with a goat biotin polyconal capture antibodies.
Cross reactionthe ELISA does not cross react with any other proteins except from closely related species
Target of this ELISANatural and recombinant mouse Syndecan-3
ELISA kit detection limits156pg/ml to 10000pg/ml
Gene testedmurine Syndecan-3
Anti-coagulationheparin or EDTA
AntigenNSO,A45-V387
Latin nameMus musculus
UniprotSyndecan-3
Gene namesyndecan 3
ELISA's sensitivity<10pg/ml
Gen protein identityQ64519
Minutes of TMB incubation25-30
Entrez nr.20970