About Rat SDC1(Syndecan 1) EIA Kit

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Size

96Test

Catalog no

E-EL-R0996-96T

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Details

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DetailDescription
Test principleThis EIA kit uses the Sandwich-EIA principle. The micro EIA plate provided in this kit has been pre-coated with an antibody specific to Rat SDC1. Standards or samples are added to the micro EIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDC1. You can calculate the concentration of Rat SDC1 in the samples by comparing the OD of the samples to the standard curve.
AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
DescriptionThis ELISA test kit or EIA Enzyme immuno assay is an enzyme linked immuno assay supplied as coated 96 well plates with antigen or antibody and needs to be stored at +4°C.
PropertiesMeSH E05 478 566 350 170 Enzyme-Linked Immunosorbent Assay
Tested Sample TypesSerum, Plasma, Cell supernatant
Target SynonymCD138, SDC, SYND1,Syndecan 1
Product NameRat SDC1(Syndecan 1) EIA Kit
Latin nameRattus norvegicus
Detection Range1.56~100ng/mL
Detection TypeColormetric
Sensitivity0.94ng/mL
TypeSandwich
UNIProt IDP26260
Target NameSDC1
Target SpeciesRat