About Mouse SDC4 (Syndecan 4) ELISA Kit

The following list shows the most important features of our product:

Size

96 Tests

Catalog no

EKE61787

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Details

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DetailDescription
Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse SDC4 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse SDC4 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse SDC4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Mouse SDC4. The concentration of Mouse SDC4 in samples can be calculated by comparing the OD of the samples to the standard curve.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED,Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
Research AreaCancer, Neuroscience, Signal transduction, Tags & Cell Markers
Cross ActivityNo significant cross-reactivity or interference was observed.
Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
ApplicationFor research use only. Not for diagnostic procedures.
Shelf lifeUse Mouse SDC4 (Syndecan 4) ELISA Kit before 6 months
Storage TemperatureShort term: 4°C; Long term: see manual.
Sample TypeSerum, Plasma, Biological Fluids
Estimated Turnaround Time6-11 business days
Detection Range0.31-20 ng/mL
Latin nameMus musculus
Detection MethodColorimetric
Sensitivity0.19 ng/mL
Assay Time4.5 hours
Shipping ConditionsIce packs
Assay TypeSandwich
Species ReactivityMouse
Target's alterntive nameSDC4
Target NameNA
SpecificityNA
StabilityNA
PrecisionNA