About Rat SDC1 (Syndecan 1) ELISA Kit

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96 Tests

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Test PrincipleThis ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rat SDC1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rat SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rat SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rat SDC1. The concentration of Rat SDC1 in samples can be calculated by comparing the OD of the samples to the standard curve.
AboutRats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Precaution of UseThe Stop Solution is acidic. Do not allow to contact skin or eyes.
Research AreaCancer, Metabolism, Neuroscience, Signal transduction, Stem cells
Cross ActivityNo significant cross-reactivity or interference was observed.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality SystemsThe kit is manufactured at ISO 9001 certified facilities.
ApplicationFor research use only. Not for diagnostic procedures.
Shelf lifeUse Rat SDC1 (Syndecan 1) ELISA Kit before 6 months
Storage TemperatureShort term: 4°C; Long term: see manual.
Sample TypeSerum, Plasma, Biological Fluids
Estimated Turnaround Time6-11 business days
Latin nameRattus norvegicus
Detection Range1.56-100 ng/mL
Detection MethodColorimetric
Sensitivity0.94 ng/mL
Shipping ConditionsIce packs
Assay Time4.5 hours
Assay TypeSandwich
Target's alterntive nameSDC1
Species ReactivityRat
Target NameNA