About Rabbit SDC1 (Syndecan 1) ELISA Kit

The following list shows the most important features of our product:

Size

96 Tests

Catalog no

EKE62489

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Details

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Detail Description
Test Principle This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Rabbit SDC1 . Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Rabbit SDC1 and Avidin-Horseradish Peroxidase (HRP) conjugate are added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Rabbit SDC1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by adding Stop Solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The OD value is proportional to the concentration of Rabbit SDC1. The concentration of Rabbit SDC1 in samples can be calculated by comparing the OD of the samples to the standard curve.
About Rabbits are used for polyclonal antibody production by Biomatik. Rabbit antibodies are very stable and can be stored for several days at room temperature. Biomatik adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Properties E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays
Precaution of Use The Stop Solution is acidic. Do not allow to contact skin or eyes.
Research Area Cancer, Metabolism, Neuroscience, Signal transduction, Stem cells
Cross Activity No significant cross-reactivity or interference was observed.
Test ELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Quality Systems The kit is manufactured at ISO 9001 certified facilities.
Shelf life Use Rabbit SDC1 (Syndecan 1) ELISA Kit before 6 months
Application For research use only. Not for diagnostic procedures.
Storage Temperature Short term: 4°C; Long term: see manual.
Sample Type Serum, Plasma, Biological Fluids
Latin name Oryctolagus cuniculus
Estimated Turnaround Time 6-11 business days
Detection Range 0.16-10 ng/mL
Detection Method Colorimetric
Shipping Conditions Ice packs
Assay Time 4.5 hours
Sensitivity 0.1 ng/mL
Assay Type Sandwich
Species Reactivity Rabbit
Target's alterntive name SDC1
Target Name NA
Specificity NA
Stability NA
Precision NA