About Syndecan 2 Antibody (HSPG)

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Size

0.1mg

Catalog no

R30996

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Details

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DetailDescription
DescriptionSyndecan-2, also known as SYND2, HSPG1, HSPG, and Fibroglycan, is a protein that in humans is encoded by the SDC2 gene. The protein encoded by this gene is a transmembrane(type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. By Southern hybridization to a panel of human-mouse somatic cell hybrid DNA and by in situ hybridization, Marynen et al.(1989) showed that the heparan sulfate proteoglycan core protein maps to 8q22-q24.(Heparan sulfate proteoglycan of basement membrane appears to be encoded by a gene on human chromosome 1.Bobardt et al.(2003) demonstrated that syndecans, including SDC2, can function as in trans HIV receptors via binding of HIV-1 gp120 to the syndecan heparan sulfate chains. Flow cytometric analysis demonstrated SDC expression on endothelial cells. HIV bound to SDC on endothelial cell lines maintained its infectivity for at least 1 week, compared with less than 1 day for unbound virus. Bobardt et al.(2003) suggested that SDC-rich endothelial cells lining the vasculature can provide a microenvironment that boosts HIV replication in T cells.
StorageAfter reconstitution, the Syndecan 2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
NotesThe stated application concentrations are suggested starting amounts. Titration of the Syndecan 2 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Species reactivityHuman (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
ImmunogenAn amino acid sequence from the middle region of human Syndecan 2/HSPG (EETDKEKVHLSDSERKMD) was used as the immunogen for this Syndecan 2 antibody.
PropertiesIf you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Intented useThis Syndecan 2 antibodyis to be used only for research purposes and not for diagnostics..
Recommended dilutionsWestern blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,IHC (Frozen): 0.5-1ug/ml
Concentration0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host animalRabbit (Oryctolagus cuniculus)
ClonalityPolyclonal (rabbit origin)
FormAntigen affinity purified
ClonePolyclonal antibody
Recognised antigenSyndecan 2 (HSPG)
Tested applicationsWB, IHC-P, IHC-F
PurityAntigen affinity
ConjugationUnconjugated
French translationanticorps
CategoryAntibody
Uniprot #P34741