About Human SDC1(Syndecan 1) ELISA Kit

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Size

96T

Catalog no

ELK2716

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Details

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DetailDescription
Test principleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 1 (SDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 1 (SDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 1 (SDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 1 (SDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
PropertiesE05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,E05 478 566 350 170 or Enzyme-Linked Immunosorbent Assays,Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
TestELISA Enzyme-linked immunosorbent assays Code 90320007 SNOMED
Research AreaTumor immunity;Infection immunity;Immunodeficiency;
Alternative NamesCD138; SDC; SYND1; Syndecan Proteoglycan 1
Detection range1.56-100ng/mL
Sensitivity0.66ng/mL
Assay TypeSandwich
Standard100ng/mL
Assay length3h