About SDC1 Polyclonal Antibody, FITC Conjugated

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Size

50 µg

Catalog no

A56801-050

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Details

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DetailDescription
PropertiesIf you buy Antibodies supplied by EpiGentek they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This EpiGentek Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. EpiGentek FITC is highly sensitive to pH extremes.
AboutPolyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
GroupPolyclonals and antibodies
ConjugationAnti-FITC Antibody
French translationanticorps